SME
Biochemistry · Problem type
Which kind of inhibitor?
Use this for “With the inhibitor, Kₘ doubled but Vₘₐₓ did not change. What kind of inhibitor is it?”
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Which kind of inhibitor does this describe?
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Ask what happens to Kₘ and Vₘₐₓ: Kₘ alone rises, both fall together, or Vₘₐₓ alone falls.
Assumptions
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All three bind reversibly; α = 1 + [I] ÷ Kᵢ is the factor each one changes Kₘ or Vₘₐₓ by.
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On a Lineweaver–Burk plot (1/v against 1/[S]) the y-intercept is 1/Vₘₐₓ and the x-intercept is −1/Kₘ.
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Noncompetitive here is the pure kind, binding the enzyme and the complex equally; mixed inhibitors fall between.
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Related lessons
Enzyme kinetics (Michaelis–Menten)
Main lesson
Kₘ and Vₘₐₓ from two rates (Lineweaver–Burk)
Use this for “An enzyme runs at 2.5 μM/s with 20 μM substrate and 4 μM/s with 80 μM. Find Kₘ and Vₘₐₓ.”
Rate with an inhibitor
Use this for “A competitive inhibitor with Kᵢ = 5 μM is added at 10 μM to an enzyme with Vₘₐₓ = 5 μM/s and Kₘ = 20 μM. Find the rate at 60 μM substrate.”