Biochemistry

Metabolic pathways

Use this for “List the enzymes of glycolysis in order. At which steps is ATP spent or made, and what is the net gain?”

Put in order

Put the steps of glycolysis in order, from glucose to pyruvate.
1
?
2
?
3
?
4
?
5
?
6
?
7
?
8
?
9
?
10
?

Assumptions

•
Counted per glucose: aldolase splits the six-carbon sugar in two, so steps 6–10 run twice.
•
Two ATP are spent (hexokinase, phosphofructokinase-1) and four are made (phosphoglycerate kinase, pyruvate kinase): 2 ATP net, with 2 NADH.
•
Each card draws the carbons as dots and the phosphates in orange; the ATP the step uses or makes shows under it once it is placed.
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